First report of using Trichoderma longibrachiatum as a biocontrol agent against Macrophomina pseudophaseolina causing charcoal rot disease of lentil in Algeria

Abstract Background Lentil ( Lens culinaris Medik.) is one of the major pulse crops in world. The present study aimed to evaluate effect Trichoderma isolates control charcoal rot disease caused by Macrophomina pseudophaseolina recently reported on lentil plants. Results In this study, antagonistic effects seven spp. isolated from rhizosphere were evaluated vitro against M1. All significantly reduced mycelial growth M1 vitro. Maximum reduction pathogen was observed at T10 (72.53%), followed T5 (63.7%) and T6 (62.88%), while T2 minimum (37.33%). Likewise, size number microsclerotia. produced microsclerotia presence T10, T1 causing 68.56 53.37% over control, respectively. maximum microsclerotia’s (54.92%), (49.84) T8 (48.68%). also revealed that production volatile metabolite, components inhibition test metabolites varied among different . most compounds, T3 41.66, 33.88, 30.55 30.37%, efficacy biocontrol agents vivo. Seed treatment with protected seedlings M. as compared untreated plants improved their growth. isolate identified based molecular tools, using internal transcribed spaces Translation elongation factor ribosomal DNA. results longibrachiatum accession MW818102 OK500004 deposited NCBI GenBank database. Conclusion first report a promising biological agent lentil.